Production of Anthocyanins in Callus Cultures of Angelica archangelica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10388268" target="_blank" >RIV/00216208:11160/18:10388268 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production of Anthocyanins in Callus Cultures of Angelica archangelica

Popis výsledku v původním jazyce

Anthocyanins have been used as food color additives, but they also possess many properties beneficial to health. Plant tissue culture technology is an attractive alternative for obtaining these valuable natural pigments. In this work, dark-grown anthocyanin producing callus cultures of Angelica archangelica were established. They were cultured on a Murashige and Skoog medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.4 mg/L benzylaminopurine. Anthocyanin contents in cultures were around 2%, i.e. one order of magnitude higher than in the intact plant that contains up to 0.17% anthocyanins. Growth and production characteristics of the culture were determined - fresh and dry biomass as well as anthocyanin levels reached a maximum on day 30. Effects of basal nutrient media on callus proliferation and anthocyanin accumulation were tested. Culture growth (fresh weight) achieved 105%, 102%, 141%, 129%, 54%, and 26%, and anthocyanin contents attained 114%, 41%, 33%, 31%, 25%, and 15% on Linsmaier and Skoog, Gamborg B5, Schenk and Hildebrandt, Woody plant, Nitsch and Nitsch, and Heller medium, respectively, in comparison with that of Murashige and Skoog.

Název v anglickém jazyce

Production of Anthocyanins in Callus Cultures of Angelica archangelica

Popis výsledku anglicky

Anthocyanins have been used as food color additives, but they also possess many properties beneficial to health. Plant tissue culture technology is an attractive alternative for obtaining these valuable natural pigments. In this work, dark-grown anthocyanin producing callus cultures of Angelica archangelica were established. They were cultured on a Murashige and Skoog medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.4 mg/L benzylaminopurine. Anthocyanin contents in cultures were around 2%, i.e. one order of magnitude higher than in the intact plant that contains up to 0.17% anthocyanins. Growth and production characteristics of the culture were determined - fresh and dry biomass as well as anthocyanin levels reached a maximum on day 30. Effects of basal nutrient media on callus proliferation and anthocyanin accumulation were tested. Culture growth (fresh weight) achieved 105%, 102%, 141%, 129%, 54%, and 26%, and anthocyanin contents attained 114%, 41%, 33%, 31%, 25%, and 15% on Linsmaier and Skoog, Gamborg B5, Schenk and Hildebrandt, Woody plant, Nitsch and Nitsch, and Heller medium, respectively, in comparison with that of Murashige and Skoog.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Natural Product Communications

ISSN

1934-578X

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

1645-1648

Kód UT WoS článku

000456230200021

EID výsledku v databázi Scopus

2-s2.0-85059559625