Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10393264" target="_blank" >RIV/00216208:11160/18:10393264 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201700288" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201700288</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201700288" target="_blank" >10.1002/elps.201700288</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Popis výsledku v původním jazyce

A sensitive method using CZE-UV detection has been developed for the determination of five tetracycline antibiotics in human urine samples. To improve the sensitivity of the method, an on-line preconcentration strategy, named field-amplified sample injection, has been developed, based on the electrokinetic injection of the sample, which requires only a 1:100 dilution with sample solvent before injection. Under optimum conditions, sensitivity enhancement factors ranged from 450 to 800 for the studied compounds. The applicability of the proposed method was demonstrated by the determination of these antibiotics in spiked urine samples. The limits of quantification were lower than 0.8 mg/L and the precision (intra- and inter-day), expressed as %RSD was below 14%. Recoveries ranged from 92.1 to 96.7%. Thus, the proposed procedure is a simple, fast and efficient strategy which could be used as therapeutic drug monitoring in human urine samples.

Název v anglickém jazyce

Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Popis výsledku anglicky

A sensitive method using CZE-UV detection has been developed for the determination of five tetracycline antibiotics in human urine samples. To improve the sensitivity of the method, an on-line preconcentration strategy, named field-amplified sample injection, has been developed, based on the electrokinetic injection of the sample, which requires only a 1:100 dilution with sample solvent before injection. Under optimum conditions, sensitivity enhancement factors ranged from 450 to 800 for the studied compounds. The applicability of the proposed method was demonstrated by the determination of these antibiotics in spiked urine samples. The limits of quantification were lower than 0.8 mg/L and the precision (intra- and inter-day), expressed as %RSD was below 14%. Recoveries ranged from 92.1 to 96.7%. Thus, the proposed procedure is a simple, fast and efficient strategy which could be used as therapeutic drug monitoring in human urine samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

608-615

Kód UT WoS článku

000425018200005

EID výsledku v databázi Scopus

2-s2.0-85036499418