Development of a capillary electrophoresis method for the separation of flavonolignans in silymarin complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F22%3A10449377" target="_blank" >RIV/00216208:11160/22:10449377 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=aFda9H6T5x" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=aFda9H6T5x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.202100212" target="_blank" >10.1002/elps.202100212</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of a capillary electrophoresis method for the separation of flavonolignans in silymarin complex

Popis výsledku v původním jazyce

CE method for the baseline separation of structurally similar flavonolignans silybin A, silybin B, isosilybin A, isosilybin B, silychristin, silydianin, and their precursor taxifolin in silymarin complex has been developed and validated. The optimized background electrolyte was 100 mmol/L boric acid (pH 9.0) containing 5 mmol/L heptakis(2,3,6-tri-O-methyl)-beta-CD and 10% (v/v) of methanol. The separation was carried out in an 80.5/72 cm (50 mu m id) fused silica capillary at +25 kV with UV detection at 200 nm. Genistein (10 mu g/mL) was used as internal standard. The resolution between the diastereomers of silybin and isosilybin was 1.73 and 2.59, respectively. The method was validated for each analyte in a concentration range of 2.5-50 mu g/mL. The calibration curves were rectilinear with correlation coefficients &gt;= 0.9972. The method was applied to determine flavonolignans in two dietary supplements containing Silybum marianum extract. The accuracy was evaluated by comparing the results of the CE analyses of the dietary supplements with those of the reference United States Pharmacopeial HPLC method. The unpaired t-test did not show a statistically significant difference between the results of both the proposed CE and the reference method (p &gt; 0.05, n = 3).

Název v anglickém jazyce

Development of a capillary electrophoresis method for the separation of flavonolignans in silymarin complex

Popis výsledku anglicky

CE method for the baseline separation of structurally similar flavonolignans silybin A, silybin B, isosilybin A, isosilybin B, silychristin, silydianin, and their precursor taxifolin in silymarin complex has been developed and validated. The optimized background electrolyte was 100 mmol/L boric acid (pH 9.0) containing 5 mmol/L heptakis(2,3,6-tri-O-methyl)-beta-CD and 10% (v/v) of methanol. The separation was carried out in an 80.5/72 cm (50 mu m id) fused silica capillary at +25 kV with UV detection at 200 nm. Genistein (10 mu g/mL) was used as internal standard. The resolution between the diastereomers of silybin and isosilybin was 1.73 and 2.59, respectively. The method was validated for each analyte in a concentration range of 2.5-50 mu g/mL. The calibration curves were rectilinear with correlation coefficients &gt;= 0.9972. The method was applied to determine flavonolignans in two dietary supplements containing Silybum marianum extract. The accuracy was evaluated by comparing the results of the CE analyses of the dietary supplements with those of the reference United States Pharmacopeial HPLC method. The unpaired t-test did not show a statistically significant difference between the results of both the proposed CE and the reference method (p &gt; 0.05, n = 3).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/EF15_003%2F0000465" target="_blank" >EF15_003/0000465: Vytvoření expertního týmu pro pokročilý výzkum v separačních vědách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

1522-2683

Svazek periodika

43

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

930-938

Kód UT WoS článku

000723121700001

EID výsledku v databázi Scopus

2-s2.0-85119967879