The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F24%3A10481712" target="_blank" >RIV/00216208:11160/24:10481712 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/24:10481712

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jp6_eQCg4X" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jp6_eQCg4X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.110170" target="_blank" >10.1016/j.microc.2024.110170</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum

Popis výsledku v původním jazyce

As a representative of the lipophilic vitamins, vitamin K is one of the most difficult vitamins to analyze. Even modern approaches using mass spectrometry detection cannot be carried out without multi-step, time-consuming, economically, and ecologically demanding sample treatment. These findings suggest an opportunity to develop new modern trends that could shorten and simplify the whole procedure, whereby emphasizing the processing of a large number of samples with an additional focus on more environmentally friendly techniques. We selected and compared the main types of both, modern and conventional approaches for vitamin K analysis in human serum including liquid-liquid extraction and solid-phase extraction on the one hand, as well as supported liquid extraction, PhreeTM phospholipid removal plate, miniaturized liquid-liquid extraction, and dispersive pipette extraction on the other hand. The different techniques were compared from an analytical perspective, including parameters such as recovery, limits of detection, and matrix effects, as well as from a non-analytical perspective, including time consumption, cost effectiveness, and manual labor required to perform the procedure. The modern techniques seem to have great advantages. Our results indicate that the PhreeTM phospholipid removal plate, and the miniaturized version of the liquid-liquid extraction are the best techniques. This work was also carried out to provide a guide for the extraction of vitamin K while determining it using the liquid chromatography-mass spectrometry technique, which may improve sample handling and help to remove potential obstacles in the future.

Název v anglickém jazyce

The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum

Popis výsledku anglicky

As a representative of the lipophilic vitamins, vitamin K is one of the most difficult vitamins to analyze. Even modern approaches using mass spectrometry detection cannot be carried out without multi-step, time-consuming, economically, and ecologically demanding sample treatment. These findings suggest an opportunity to develop new modern trends that could shorten and simplify the whole procedure, whereby emphasizing the processing of a large number of samples with an additional focus on more environmentally friendly techniques. We selected and compared the main types of both, modern and conventional approaches for vitamin K analysis in human serum including liquid-liquid extraction and solid-phase extraction on the one hand, as well as supported liquid extraction, PhreeTM phospholipid removal plate, miniaturized liquid-liquid extraction, and dispersive pipette extraction on the other hand. The different techniques were compared from an analytical perspective, including parameters such as recovery, limits of detection, and matrix effects, as well as from a non-analytical perspective, including time consumption, cost effectiveness, and manual labor required to perform the procedure. The modern techniques seem to have great advantages. Our results indicate that the PhreeTM phospholipid removal plate, and the miniaturized version of the liquid-liquid extraction are the best techniques. This work was also carried out to provide a guide for the extraction of vitamin K while determining it using the liquid chromatography-mass spectrometry technique, which may improve sample handling and help to remove potential obstacles in the future.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

<a href="/cs/project/NU21J-02-00021" target="_blank" >NU21J-02-00021: Rozdíly v parametrech agregace krevních destiček a koagulace krve mezi zdravými osobami a pacienty s metabolickými chorobami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

198

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

110170

Kód UT WoS článku

001203047400001

EID výsledku v databázi Scopus

2-s2.0-85187300798