Phe475 and Glu446 but not Ser445 participate in ATP-binding to the alfa-subunit of NA/K-ATPase

Kód výsledku v IS VaVaI

RIV/00216208:11310/02:00007337 - isvavai.cz

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phe475 and Glu446 but not Ser445 participate in ATP-binding to the alfa-subunit of NA/K-ATPase

Popis výsledku v původním jazyce

The ATP-binding site of Na+/K+-ATPase is localized on the large cytoplasmic loop of the small alpha, Greek-subunit between transmembrane helices H4 and H5. Site-directed mutagenesis was performed to identify residues involved in ATP binding. On the basisof our recently developed model of this loop, Ser445, Glu446, and Phe475 were proposed to be close to the binding pocket. Replacement of Phe475 with Trp and Glu446 with Gln profoundly reduced the binding of ATP, whereas the substitution of Ser445 with Ala did not affect ATP binding. Fluorescence measurements of the fluorescent analog TNP-ATP, however, indicated that Ser445 is close to the binding site, although it does not participate in binding

Název v anglickém jazyce

Phe475 and Glu446 but not Ser445 participate in ATP-binding to the alfa-subunit of NA/K-ATPase

Popis výsledku anglicky

The ATP-binding site of Na+/K+-ATPase is localized on the large cytoplasmic loop of the small alpha, Greek-subunit between transmembrane helices H4 and H5. Site-directed mutagenesis was performed to identify residues involved in ATP binding. On the basisof our recently developed model of this loop, Ser445, Glu446, and Phe475 were proposed to be close to the binding pocket. Replacement of Phe475 with Trp and Glu446 with Gln profoundly reduced the binding of ATP, whereas the substitution of Ser445 with Ala did not affect ATP binding. Fluorescence measurements of the fluorescent analog TNP-ATP, however, indicated that Ser445 is close to the binding site, although it does not participate in binding

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

LN00A026: Centrum molekulární a buněčné imunologie

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical and Biophysical Research Communications

ISSN

0006-291X

e-ISSN

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Svazek periodika

297

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

154-159

Kód UT WoS článku

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EID výsledku v databázi Scopus

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