Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F07%3A13683" target="_blank" >RIV/00216208:11310/07:13683 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein

Popis výsledku v původním jazyce

The response of transgenic Nicotiana tabacum L. plants carrying the gene for potyviral non-structural P3 protein to Potato virus Y (strain NTN) infection was not significantly different from the wild type. The activities of antioxidant enzymes catalase (CAT), glutathion reductase (GR), ascorbate peroxidase (APOD) and superoxid dismutase (SOD), determined in systemically infected leaves at early stages of the infection did not change except for the transient increase in CAT and GR activities in the wildtype infected plants compared to the healthy ones. At the stages, when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several enzymes of anaplerotic metabolic pathways, NADP-dependent malic enzyme (NADP-ME), phosphoenolpyruvate carboxylase (PEPC), and pyruvate, orthophosphate dikinase (PPDK) in transgenic plants was found, similarly as in the wild type.

Název v anglickém jazyce

Effect of Potato virus Y on the activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum L. transgenic plants transformed with the gene for P3 protein

Popis výsledku anglicky

The response of transgenic Nicotiana tabacum L. plants carrying the gene for potyviral non-structural P3 protein to Potato virus Y (strain NTN) infection was not significantly different from the wild type. The activities of antioxidant enzymes catalase (CAT), glutathion reductase (GR), ascorbate peroxidase (APOD) and superoxid dismutase (SOD), determined in systemically infected leaves at early stages of the infection did not change except for the transient increase in CAT and GR activities in the wildtype infected plants compared to the healthy ones. At the stages, when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several enzymes of anaplerotic metabolic pathways, NADP-dependent malic enzyme (NADP-ME), phosphoenolpyruvate carboxylase (PEPC), and pyruvate, orthophosphate dikinase (PPDK) in transgenic plants was found, similarly as in the wild type.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0505" target="_blank" >1M0505: Centrum cílených terapeutik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

General and Applied Plant Physiology

ISSN

1312-8183

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

19

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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