Probing plant membranes with FM dyes: tracking, dragging or blocking?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F10%3A10000449" target="_blank" >RIV/00216208:11310/10:10000449 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/10:00343962

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Probing plant membranes with FM dyes: tracking, dragging or blocking?

Popis výsledku v původním jazyce

The goal of this manuscript is to describe unexplored effects of the FM styryl dyes, which are widely used probes that label processes of vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor bycytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using

Název v anglickém jazyce

Probing plant membranes with FM dyes: tracking, dragging or blocking?

Popis výsledku anglicky

The goal of this manuscript is to describe unexplored effects of the FM styryl dyes, which are widely used probes that label processes of vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor bycytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Journal

ISSN

0960-7412

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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