Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10099223" target="_blank" >RIV/00216208:11310/11:10099223 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.molbiopara.2010.12.011" target="_blank" >http://dx.doi.org/10.1016/j.molbiopara.2010.12.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2010.12.011" target="_blank" >10.1016/j.molbiopara.2010.12.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes

Popis výsledku v původním jazyce

The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T.vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome land II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mappingof the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.

Název v anglickém jazyce

Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes

Popis výsledku anglicky

The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T.vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome land II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mappingof the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC07032" target="_blank" >LC07032: Centrum funkční genetiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

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Svazek periodika

176

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

135-137

Kód UT WoS článku

000288629800011

EID výsledku v databázi Scopus

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