Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10100566" target="_blank" >RIV/00216208:11310/11:10100566 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/11:00370402

Výsledek na webu

<a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >http://dx.doi.org/10.1155/2011/634253</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >10.1155/2011/634253</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

Popis výsledku v původním jazyce

The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHC? was represented by the lower band with higher mobility and MyHC? by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHC? possessed a higher mobility rate than the MyHC? isoform. Our data highlighted the importance of the careful identification of the MyHC? and MyHC? isoforms analyzed by the SDS-PAGE.

Název v anglickém jazyce

Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

Popis výsledku anglicky

The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHC? was represented by the lower band with higher mobility and MyHC? by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHC? possessed a higher mobility rate than the MyHC? isoform. Our data highlighted the importance of the careful identification of the MyHC? and MyHC? isoforms analyzed by the SDS-PAGE.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomedicine and Biotechnology

ISSN

1110-7243

e-ISSN

—

Svazek periodika

2011

Číslo periodika v rámci svazku

—

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000298749900001

EID výsledku v databázi Scopus

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