Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10108322" target="_blank" >RIV/00216208:11310/11:10108322 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/11:00371215

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mimet.2011.08.012" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2011.08.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2011.08.012" target="_blank" >10.1016/j.mimet.2011.08.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium

Popis výsledku v původním jazyce

Protozoan hemoflagellates Leishmania are causative agents of leishmaniases and an important biological model for study of host-pathogen interaction. A wide range of methods of Leishmania cultivation on both biphasic and liquid media is available. Biphasic media are considered to be superior for initial isolation of the parasites and obtaining high promastigote infectivity; however, liquid media are more suitable for large-scale experiments. The aim of the present study was the adaptation and optimization of the cultivation of Leishmania promastigotes on a biphasic SNB-9 (saline-neopeptone-blood 9) medium that was originally developed for Trypanosoma cultivation and combines the advantages of biphasic and liquid media. SNB-9 medium is characterized witha large volume of the liquid phase, which facilitates the manipulation with the culture and provides parasite yields comparable to parasite yields on such liquid medium as Schneider&amp;amp;apos;s Insect Medium. We demonstrate that SNB-9

Název v anglickém jazyce

Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium

Popis výsledku anglicky

Protozoan hemoflagellates Leishmania are causative agents of leishmaniases and an important biological model for study of host-pathogen interaction. A wide range of methods of Leishmania cultivation on both biphasic and liquid media is available. Biphasic media are considered to be superior for initial isolation of the parasites and obtaining high promastigote infectivity; however, liquid media are more suitable for large-scale experiments. The aim of the present study was the adaptation and optimization of the cultivation of Leishmania promastigotes on a biphasic SNB-9 (saline-neopeptone-blood 9) medium that was originally developed for Trypanosoma cultivation and combines the advantages of biphasic and liquid media. SNB-9 medium is characterized witha large volume of the liquid phase, which facilitates the manipulation with the culture and provides parasite yields comparable to parasite yields on such liquid medium as Schneider&amp;amp;apos;s Insect Medium. We demonstrate that SNB-9

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiological Methods

ISSN

0167-7012

e-ISSN

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Svazek periodika

87

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

273-277

Kód UT WoS článku

000297892200003

EID výsledku v databázi Scopus

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