Comparison of LC Columns Packed with 2.6 mu m Core-Shell and Sub-2 mu m Porous Particles for Gradient Separation of Antibiotics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10112536" target="_blank" >RIV/00216208:11310/11:10112536 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-011-2021-7" target="_blank" >http://dx.doi.org/10.1007/s10337-011-2021-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-011-2021-7" target="_blank" >10.1007/s10337-011-2021-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of LC Columns Packed with 2.6 mu m Core-Shell and Sub-2 mu m Porous Particles for Gradient Separation of Antibiotics

Popis výsledku v původním jazyce

The recently introduced Kinetex C18 column packed with core-shell 2.6 mu m particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 mu m porous particles. Unlike Acquity BEH C18 column, Kinetex C18 columnexhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 x 50 mm) and Acquity BEH C18 column (2.1 x 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). This indicated that Kinetex C18 was a suitab

Název v anglickém jazyce

Comparison of LC Columns Packed with 2.6 mu m Core-Shell and Sub-2 mu m Porous Particles for Gradient Separation of Antibiotics

Popis výsledku anglicky

The recently introduced Kinetex C18 column packed with core-shell 2.6 mu m particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 mu m porous particles. Unlike Acquity BEH C18 column, Kinetex C18 columnexhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 x 50 mm) and Acquity BEH C18 column (2.1 x 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). This indicated that Kinetex C18 was a suitab

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/1M06011" target="_blank" >1M06011: Centrum molekulárních metod monitorování difúzního znečištění životního prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

—

Svazek periodika

74

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

19-27

Kód UT WoS článku

000291745400003

EID výsledku v databázi Scopus

—