Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10120331" target="_blank" >RIV/00216208:11310/12:10120331 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/12:00381851 RIV/61388955:_____/12:00381851

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0005273612001538" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0005273612001538</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2012.05.005" target="_blank" >10.1016/j.bbamem.2012.05.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

Popis výsledku v původním jazyce

Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein,namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p.

Název v anglickém jazyce

Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

Popis výsledku anglicky

Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein,namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Biomembranes

ISSN

0005-2736

e-ISSN

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Svazek periodika

1818

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

2126-2134

Kód UT WoS článku

000306882600006

EID výsledku v databázi Scopus

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