Simulation of the effects of complex- formation equilibria in electrophoresis: II. Experimental verification
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10120348" target="_blank" >RIV/00216208:11310/12:10120348 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elps.201100503" target="_blank" >http://dx.doi.org/10.1002/elps.201100503</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201100503" target="_blank" >10.1002/elps.201100503</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Simulation of the effects of complex- formation equilibria in electrophoresis: II. Experimental verification
Popis výsledku v původním jazyce
The complete mathematical model of electromigration in systems with complexation agents introduced in the Part I of this article (V. Hruska et al., Eletrophoresis, 2012, 33, this issue), which was implemented into our simulation program Simul 5, was verified experimentally. Three different chiral selector (CS) systems differing in the type of the CS, the magnitude of the complexation constants as well as in the experimental conditions were selected for verification. The experiments and simulations wereperformed at various concentrations of the CSs in order to discuss the influence of the concentration of the CS on the separation. The simulated and experimental electropherograms show very good agreement in the position, shape and amplitude of the analyte peaks. The new Simul 5 Complex offers a deep insight into electrophoretical separations that take place in systems containing complexing agents, for example into enantiomer separations. Using Simul 5 Complex we were able to predict and
Název v anglickém jazyce
Simulation of the effects of complex- formation equilibria in electrophoresis: II. Experimental verification
Popis výsledku anglicky
The complete mathematical model of electromigration in systems with complexation agents introduced in the Part I of this article (V. Hruska et al., Eletrophoresis, 2012, 33, this issue), which was implemented into our simulation program Simul 5, was verified experimentally. Three different chiral selector (CS) systems differing in the type of the CS, the magnitude of the complexation constants as well as in the experimental conditions were selected for verification. The experiments and simulations wereperformed at various concentrations of the CSs in order to discuss the influence of the concentration of the CS on the separation. The simulated and experimental electropherograms show very good agreement in the position, shape and amplitude of the analyte peaks. The new Simul 5 Complex offers a deep insight into electrophoretical separations that take place in systems containing complexing agents, for example into enantiomer separations. Using Simul 5 Complex we were able to predict and
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA203%2F08%2F1428" target="_blank" >GA203/08/1428: Kapilární elektroforéza s multidimenzionální detekcí a její užití pro separaci a charakterizaci biopolymerů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrophoresis
ISSN
0173-0835
e-ISSN
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Svazek periodika
33
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
948-957
Kód UT WoS článku
000303155700009
EID výsledku v databázi Scopus
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