A nonlinear electrophoretic model for PeakMaster: Part III. Electromigration dispersion in systems that contain a neutral complex-forming agent and a fully charged analyte. Theory

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10124648" target="_blank" >RIV/00216208:11310/12:10124648 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2012.06.086" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2012.06.086</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2012.06.086" target="_blank" >10.1016/j.chroma.2012.06.086</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A nonlinear electrophoretic model for PeakMaster: Part III. Electromigration dispersion in systems that contain a neutral complex-forming agent and a fully charged analyte. Theory

Popis výsledku v původním jazyce

We introduce a new nonlinear electrophoretic model for complex-forming systems with a fully charged analyte and a neutral ligand. The background electrolyte is supposed to be composed of two constituents, which do not interact with the ligand. In order to characterize the electromigration dispersion (EMD) of the analyte zone we define a new parameter, the nonlinear electromigration mobility slope, SEMD,A. The parameter can be easily utilized for quantitative prediction of the EMD and for comparisons ofthe model with the simulated and experimental profiles. We implemented the model to the new version of Peak- Master 5.3 Complex that can calculate some characteristic parameters of the electrophoretic system and can plot the dependence of SEMD,A on the concentration of the ligand. Besides SEMD,A, also the relative velocity slope, SX, can be calculated. It is commonly used as a measure of EMD in electrophoretic systems. PeakMaster 5.3 Complex software can be advantageously used for optimi

Název v anglickém jazyce

A nonlinear electrophoretic model for PeakMaster: Part III. Electromigration dispersion in systems that contain a neutral complex-forming agent and a fully charged analyte. Theory

Popis výsledku anglicky

We introduce a new nonlinear electrophoretic model for complex-forming systems with a fully charged analyte and a neutral ligand. The background electrolyte is supposed to be composed of two constituents, which do not interact with the ligand. In order to characterize the electromigration dispersion (EMD) of the analyte zone we define a new parameter, the nonlinear electromigration mobility slope, SEMD,A. The parameter can be easily utilized for quantitative prediction of the EMD and for comparisons ofthe model with the simulated and experimental profiles. We implemented the model to the new version of Peak- Master 5.3 Complex that can calculate some characteristic parameters of the electrophoretic system and can plot the dependence of SEMD,A on the concentration of the ligand. Besides SEMD,A, also the relative velocity slope, SX, can be calculated. It is commonly used as a measure of EMD in electrophoretic systems. PeakMaster 5.3 Complex software can be advantageously used for optimi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F08%2F1428" target="_blank" >GA203/08/1428: Kapilární elektroforéza s multidimenzionální detekcí a její užití pro separaci a charakterizaci biopolymerů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1267

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

102-108

Kód UT WoS článku

000311658100012

EID výsledku v databázi Scopus

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