Loss of membrane fluidity and endocytosis inhibition are involved in rapid aluminum-induced root growth cessation in Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10124874" target="_blank" >RIV/00216208:11310/12:10124874 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/12:00380983 RIV/60461373:22330/12:43894182

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0981942812002094" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0981942812002094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2012.07.030" target="_blank" >10.1016/j.plaphy.2012.07.030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Loss of membrane fluidity and endocytosis inhibition are involved in rapid aluminum-induced root growth cessation in Arabidopsis thaliana

Popis výsledku v původním jazyce

Aluminum (Al) toxicity is the main limiting factor in crop production on acid soils. The main symptom of Al toxicity is a rapid inhibition of root growth, but the mechanism of root growth cessation remains unclear. Here we examined the earliest changes in the plasma membrane and processes related to the membrane in the Arabidopsis thaliana root tip cells of roots grown in a hydropony. Al suppressed root growth within 2 min, inhibited endocytosis within 10 min of exposure and stabilized cortical microtubules within the first 30 min. Spectrofluorometric measurements of the plasma membrane isolated from Arabidopsis plants and labeled with the fluorescent probe laurdan showed that Al induced a reduction in membrane fluidity. Application of the membrane fluidizer, benzyl alcohol, restored partially membrane fluidity and also partially restored root growth during first 30 min of Al treatment. We concluded that Al-induced loss of membrane fluidity and endocytosis inhibition occurred very earl

Název v anglickém jazyce

Loss of membrane fluidity and endocytosis inhibition are involved in rapid aluminum-induced root growth cessation in Arabidopsis thaliana

Popis výsledku anglicky

Aluminum (Al) toxicity is the main limiting factor in crop production on acid soils. The main symptom of Al toxicity is a rapid inhibition of root growth, but the mechanism of root growth cessation remains unclear. Here we examined the earliest changes in the plasma membrane and processes related to the membrane in the Arabidopsis thaliana root tip cells of roots grown in a hydropony. Al suppressed root growth within 2 min, inhibited endocytosis within 10 min of exposure and stabilized cortical microtubules within the first 30 min. Spectrofluorometric measurements of the plasma membrane isolated from Arabidopsis plants and labeled with the fluorescent probe laurdan showed that Al induced a reduction in membrane fluidity. Application of the membrane fluidizer, benzyl alcohol, restored partially membrane fluidity and also partially restored root growth during first 30 min of Al treatment. We concluded that Al-induced loss of membrane fluidity and endocytosis inhibition occurred very earl

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

listopad

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

88-97

Kód UT WoS článku

000310387400012

EID výsledku v databázi Scopus

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