Evaluation of damage to DNA induced by UV-C radiation and chemical agents using electrochemical biosensor based on low molecular weight DNA and screen-printed carbon electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126368" target="_blank" >RIV/00216208:11310/12:10126368 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2012.03.119" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2012.03.119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2012.03.119" target="_blank" >10.1016/j.electacta.2012.03.119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of damage to DNA induced by UV-C radiation and chemical agents using electrochemical biosensor based on low molecular weight DNA and screen-printed carbon electrode

Popis výsledku v původním jazyce

There is great interest and need to detect and evaluate damage to DNA by environmental factors. In the present paper, simple electrochemical DNA biosensors composed of commercially available screen-printed carbon electrode (SPCE) and low molecular weightdouble-stranded DNA (dsDNA) recognition layer are reported and applied to the detection of damage to DNA by UV-C radiation and reactive oxygen species produced by the Fenton type reaction in model as well as mineral water samples with additives. ComplexDNA biosensor response is based on square-wave voltammetric intrinsic signal of the guanine moiety as well as that of the intercalative indicator thioridazine, cyclic voltammetric response of the [Fe(CN)(6)](3-/4-) indicator in solution and on electrochemical impedance spectroscopy when the measurements can be performed in the same solution. For the last two types of measurements, the biosensor was also used with an interface between the SPCE and DNA formed by a composite of carboxylate

Název v anglickém jazyce

Evaluation of damage to DNA induced by UV-C radiation and chemical agents using electrochemical biosensor based on low molecular weight DNA and screen-printed carbon electrode

Popis výsledku anglicky

There is great interest and need to detect and evaluate damage to DNA by environmental factors. In the present paper, simple electrochemical DNA biosensors composed of commercially available screen-printed carbon electrode (SPCE) and low molecular weightdouble-stranded DNA (dsDNA) recognition layer are reported and applied to the detection of damage to DNA by UV-C radiation and reactive oxygen species produced by the Fenton type reaction in model as well as mineral water samples with additives. ComplexDNA biosensor response is based on square-wave voltammetric intrinsic signal of the guanine moiety as well as that of the intercalative indicator thioridazine, cyclic voltammetric response of the [Fe(CN)(6)](3-/4-) indicator in solution and on electrochemical impedance spectroscopy when the measurements can be performed in the same solution. For the last two types of measurements, the biosensor was also used with an interface between the SPCE and DNA formed by a composite of carboxylate

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica Acta

ISSN

0013-4686

e-ISSN

—

Svazek periodika

71

Číslo periodika v rámci svazku

Jene

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

134-139

Kód UT WoS článku

000304510900021

EID výsledku v databázi Scopus

—