A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae From Bee Debris

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10129223" target="_blank" >RIV/00216208:11310/12:10129223 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/12:00384289

Výsledek na webu

<a href="http://www.bioone.org/doi/abs/10.1603/EC12010" target="_blank" >http://www.bioone.org/doi/abs/10.1603/EC12010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1603/EC12010" target="_blank" >10.1603/EC12010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae From Bee Debris

Popis výsledku v původním jazyce

American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use thenew method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less)

Název v anglickém jazyce

A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae From Bee Debris

Popis výsledku anglicky

American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use thenew method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less)

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

<a href="/cs/project/SP%2F2D3%2F60%2F08" target="_blank" >SP/2D3/60/08: Integrovaný výzkum vlivů a důsledků vypouštění uměle odchovaných kachen divokých na vodní a mokřadní ekosystémy s použitím moderních ekologických a genetických přístupů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Economic Entomology

ISSN

0022-0493

e-ISSN

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Svazek periodika

105

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

1487-1491

Kód UT WoS článku

000312443000002

EID výsledku v databázi Scopus

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