Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10132173" target="_blank" >RIV/00216208:11310/12:10132173 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985939:_____/12:00381075
Výsledek na webu
<a href="http://www.amjbot.org/content/99/2/e74.full.pdf+html" target="_blank" >http://www.amjbot.org/content/99/2/e74.full.pdf+html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3732/ajb.1100416" target="_blank" >10.3732/ajb.1100416</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes
Popis výsledku v původním jazyce
The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with publicDNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for otherAsteraceae tribes. All three markers had higher variation (2.1 MINUS SIGN 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, esp. for very closely related species
Název v anglickém jazyce
Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes
Popis výsledku anglicky
The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with publicDNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for otherAsteraceae tribes. All three markers had higher variation (2.1 MINUS SIGN 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, esp. for very closely related species
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
American Journal of Botany
ISSN
0002-9122
e-ISSN
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Svazek periodika
99
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
4
Strana od-do
74-77
Kód UT WoS článku
000304812600011
EID výsledku v databázi Scopus
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