Proteome and metabolome profiling of cytokinin action in Arabidopsis identifies distinct but also similar responses to cytokinin down- and up-regulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10133672" target="_blank" >RIV/00216208:11310/13:10133672 - isvavai.cz</a>

Výsledek na webu

<a href="http://jxb.oxfordjournals.org/content/early/2013/09/20/jxb.ert227.full" target="_blank" >http://jxb.oxfordjournals.org/content/early/2013/09/20/jxb.ert227.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ert227" target="_blank" >10.1093/jxb/ert227</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteome and metabolome profiling of cytokinin action in Arabidopsis identifies distinct but also similar responses to cytokinin down- and up-regulation

Popis výsledku v původním jazyce

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and Agrobacterial isopentenyltransferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development and/or hormone and light signaling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates and organic acids. Comparison of the datasets obtained from activated CaMV35S>GR>HvCKX2 and C

Název v anglickém jazyce

Proteome and metabolome profiling of cytokinin action in Arabidopsis identifies distinct but also similar responses to cytokinin down- and up-regulation

Popis výsledku anglicky

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and Agrobacterial isopentenyltransferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development and/or hormone and light signaling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates and organic acids. Comparison of the datasets obtained from activated CaMV35S>GR>HvCKX2 and C

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

4193-4206

Kód UT WoS článku

000326724700008

EID výsledku v databázi Scopus

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