Determination of 4-Aminobiphenyl and 4-Nitrobiphenyl by HPLC with Electrochemical, UV Spectrophotometric, and Fluorescent Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10134225" target="_blank" >RIV/00216208:11310/13:10134225 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol8/80202524.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol8/80202524.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of 4-Aminobiphenyl and 4-Nitrobiphenyl by HPLC with Electrochemical, UV Spectrophotometric, and Fluorescent Detection

Popis výsledku v původním jazyce

HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl -4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized. UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 x 10(-6) mol L-1, 2.0 x 10(-6) mol L-1, and 8.0 x 10(-8) mol L-1 for UV, electrochemical, and fluorescent detection, respectively. For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 x 10(-6) mol L-1 (UV), 4.0 x 10(-6) mol L-1 (electrochemical), and 2.0 x 10(-7) mol L-1 (fluorescent detection). Moreover, it was also possible to determine 4-NBP directly using HPLC with UV detection, where th

Název v anglickém jazyce

Determination of 4-Aminobiphenyl and 4-Nitrobiphenyl by HPLC with Electrochemical, UV Spectrophotometric, and Fluorescent Detection

Popis výsledku anglicky

HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl -4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized. UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 x 10(-6) mol L-1, 2.0 x 10(-6) mol L-1, and 8.0 x 10(-8) mol L-1 for UV, electrochemical, and fluorescent detection, respectively. For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 x 10(-6) mol L-1 (UV), 4.0 x 10(-6) mol L-1 (electrochemical), and 2.0 x 10(-7) mol L-1 (fluorescent detection). Moreover, it was also possible to determine 4-NBP directly using HPLC with UV detection, where th

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

12

Strana od-do

2524-2535

Kód UT WoS článku

000316565800084

EID výsledku v databázi Scopus

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