Use of a handheld Raman spectrometer for fast screening of microbial pigments in cultures of halophilic microorganisms and in microbial communities in hypersaline environments in nature

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10144022" target="_blank" >RIV/00216208:11310/13:10144022 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jrs.4362" target="_blank" >http://dx.doi.org/10.1002/jrs.4362</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.4362" target="_blank" >10.1002/jrs.4362</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of a handheld Raman spectrometer for fast screening of microbial pigments in cultures of halophilic microorganisms and in microbial communities in hypersaline environments in nature

Popis výsledku v původním jazyce

A compact handheld Raman spectrometer equipped with a 532nm excitation laser was used to detect pigments as possible biomarkers in autotrophic (cyanobacteria and purple sulfur bacteria) and heterotrophic halophilic microorganisms (Archaea of the family Halobacteriaceae, Salinibacter). Common as well as less common carotenoids, including -bacterioruberin, salinixanthin, and spirilloxanthin derivatives were detected in cell pellets of model organisms belonging to the genera Haloferax, Haloarcula, Halobacterium (Archaea), Salinibacter (Bacteroidetes), and Ectothiorhodospira (Gammaproteobacteria). Direct measurements on such cultures provide fast and reliable identification of pigments. Bacterioruberin was detected as the dominant carotenoid in pellets ofcells collected from the saltern crystallizer ponds in Eilat, Israel. Raman analysis of the colored layered microbial communities in a benthic gypsum crust in the saltern evaporation ponds showed signals consistent with the presence of my

Název v anglickém jazyce

Use of a handheld Raman spectrometer for fast screening of microbial pigments in cultures of halophilic microorganisms and in microbial communities in hypersaline environments in nature

Popis výsledku anglicky

A compact handheld Raman spectrometer equipped with a 532nm excitation laser was used to detect pigments as possible biomarkers in autotrophic (cyanobacteria and purple sulfur bacteria) and heterotrophic halophilic microorganisms (Archaea of the family Halobacteriaceae, Salinibacter). Common as well as less common carotenoids, including -bacterioruberin, salinixanthin, and spirilloxanthin derivatives were detected in cell pellets of model organisms belonging to the genera Haloferax, Haloarcula, Halobacterium (Archaea), Salinibacter (Bacteroidetes), and Ectothiorhodospira (Gammaproteobacteria). Direct measurements on such cultures provide fast and reliable identification of pigments. Bacterioruberin was detected as the dominant carotenoid in pellets ofcells collected from the saltern crystallizer ponds in Eilat, Israel. Raman analysis of the colored layered microbial communities in a benthic gypsum crust in the saltern evaporation ponds showed signals consistent with the presence of my

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP210%2F10%2F0467" target="_blank" >GAP210/10/0467: Detekce exobiologických markerů Ramanovou spektrometrií jako hlavní metodou</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

—

Svazek periodika

44

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1285-1291

Kód UT WoS článku

000323784900012

EID výsledku v databázi Scopus

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