Large-volume sample stacking for in vivo monitoring of trace levels of gamma-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10144149" target="_blank" >RIV/00216208:11310/13:10144149 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/13:43907478

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.019" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2013.06.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.019" target="_blank" >10.1016/j.chroma.2013.06.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Large-volume sample stacking for in vivo monitoring of trace levels of gamma-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection

Popis výsledku v původním jazyce

A new variant of large-volume sample stacking injection (LVSS) was used in the capillary electrophoresis with capacitively coupled contactless conductivity detection (CE/(CD)-D-4) determination of the neurotransmitters gamma-aminobutyric acid (GABA), glycine (Gly) and glutamate (Glu) in microdialysates of periaqueductal gray matter (PAG). The separation capillary was filled to 98% from the injection side with a sample of microdialysate in acetonitrile. Simultaneously with turning on the separation voltage, the sample zone was forced out by the background electrolyte by increasing the pressure in the terminal capillary outlet vessel. As a consequence of the stacking effect, the analyte was concentrated from the large sample volume into a narrow zone atthe sample/background electrolyte boundary close to the injection end of the capillary. Under these conditions, LOD values of 9, 10 and 15 nM were determined in the model samples for GABA, Gly and Glu, respectively; RSD equalled 0.5% for

Název v anglickém jazyce

Large-volume sample stacking for in vivo monitoring of trace levels of gamma-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection

Popis výsledku anglicky

A new variant of large-volume sample stacking injection (LVSS) was used in the capillary electrophoresis with capacitively coupled contactless conductivity detection (CE/(CD)-D-4) determination of the neurotransmitters gamma-aminobutyric acid (GABA), glycine (Gly) and glutamate (Glu) in microdialysates of periaqueductal gray matter (PAG). The separation capillary was filled to 98% from the injection side with a sample of microdialysate in acetonitrile. Simultaneously with turning on the separation voltage, the sample zone was forced out by the background electrolyte by increasing the pressure in the terminal capillary outlet vessel. As a consequence of the stacking effect, the analyte was concentrated from the large sample volume into a narrow zone atthe sample/background electrolyte boundary close to the injection end of the capillary. Under these conditions, LOD values of 9, 10 and 15 nM were determined in the model samples for GABA, Gly and Glu, respectively; RSD equalled 0.5% for

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1303

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

94-99

Kód UT WoS článku

000322562200012

EID výsledku v databázi Scopus

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