Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10145637" target="_blank" >RIV/00216208:11310/13:10145637 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/13:00399070

Výsledek na webu

<a href="http://dx.doi.org/10.1111/1751-7915.12007" target="_blank" >http://dx.doi.org/10.1111/1751-7915.12007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1751-7915.12007" target="_blank" >10.1111/1751-7915.12007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots

Popis výsledku v původním jazyce

Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, atranscriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17 alpha-ethinyloestradiol (EE2) degradation to study regulation mechanisms used byfungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 +/- 6.7 U g(-1) of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor,

Název v anglickém jazyce

Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots

Popis výsledku anglicky

Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, atranscriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17 alpha-ethinyloestradiol (EE2) degradation to study regulation mechanisms used byfungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 +/- 6.7 U g(-1) of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Biotechnology

ISSN

1751-7907

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

300-306

Kód UT WoS článku

000320186500010

EID výsledku v databázi Scopus

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