InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10173717" target="_blank" >RIV/00216208:11310/13:10173717 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/13:43871792

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetpar.2013.04.015" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2013.04.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2013.04.015" target="_blank" >10.1016/j.vetpar.2013.04.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus

Popis výsledku v původním jazyce

Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch (TM) TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA). is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24 h. In this study we successfully cultivated P. hominis in InPouch (TM) TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch (TM) TF-Feline medium. PCR-base

Název v anglickém jazyce

InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus

Popis výsledku anglicky

Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch (TM) TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA). is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24 h. In this study we successfully cultivated P. hominis in InPouch (TM) TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch (TM) TF-Feline medium. PCR-base

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

—

Svazek periodika

196

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

503-505

Kód UT WoS článku

000323864500036

EID výsledku v databázi Scopus

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