Deep sequencing of Trichomonas vaginalis during the early infection of vaginal epithelial cells and amoeboid transition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10189477" target="_blank" >RIV/00216208:11310/13:10189477 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijpara.2013.04.002" target="_blank" >http://dx.doi.org/10.1016/j.ijpara.2013.04.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpara.2013.04.002" target="_blank" >10.1016/j.ijpara.2013.04.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Deep sequencing of Trichomonas vaginalis during the early infection of vaginal epithelial cells and amoeboid transition

Popis výsledku v původním jazyce

The human pathogen Trichomonas vaginalis has the largest protozoan genome known, potentially encoding approximately 60,000 proteins. To what degree these genes are expressed is not well known and only a few key transcription factors and promoter domainshave been identified. To shed light on the expression capacity of the parasite and transcriptional regulation during phase transitions, we deep sequenced the transcriptomes of the protozoan during two environmental stimuli of the early infection process:exposure to oxygen and contact with vaginal epithelial cells. Eleven 3' fragment libraries from different time points after exposure to oxygen only and in combination with human tissue were sequenced, generating more than 150 million reads which mappedonto 33,157 protein coding genes in total and a core set of more than 20,000 genes represented within all libraries. The data uncover gene family expression regulation in this parasite and give evidence for a concentrated response to the

Název v anglickém jazyce

Deep sequencing of Trichomonas vaginalis during the early infection of vaginal epithelial cells and amoeboid transition

Popis výsledku anglicky

The human pathogen Trichomonas vaginalis has the largest protozoan genome known, potentially encoding approximately 60,000 proteins. To what degree these genes are expressed is not well known and only a few key transcription factors and promoter domainshave been identified. To shed light on the expression capacity of the parasite and transcriptional regulation during phase transitions, we deep sequenced the transcriptomes of the protozoan during two environmental stimuli of the early infection process:exposure to oxygen and contact with vaginal epithelial cells. Eleven 3' fragment libraries from different time points after exposure to oxygen only and in combination with human tissue were sequenced, generating more than 150 million reads which mappedonto 33,157 protein coding genes in total and a core set of more than 20,000 genes represented within all libraries. The data uncover gene family expression regulation in this parasite and give evidence for a concentrated response to the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal for Parasitology

ISSN

0020-7519

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

707-719

Kód UT WoS článku

000322344900003

EID výsledku v databázi Scopus

—