Inhibition of In Vitro Leukotriene B-4 Biosynthesis in Human Neutrophil Granulocytes and Docking Studies of Natural Quinones

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190509" target="_blank" >RIV/00216208:11310/13:10190509 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/13:00395042 RIV/60460709:41210/13:60053 RIV/60460709:41340/13:60053

Výsledek na webu

<a href="http://www.academia.edu/3513300/Inhibition_of_in_vitro_leukotriene_B4_biosynthesis_in_human_neutrophil_granulocytes_and_docking_studies_of_natural_quinones" target="_blank" >http://www.academia.edu/3513300/Inhibition_of_in_vitro_leukotriene_B4_biosynthesis_in_human_neutrophil_granulocytes_and_docking_studies_of_natural_quinones</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of In Vitro Leukotriene B-4 Biosynthesis in Human Neutrophil Granulocytes and Docking Studies of Natural Quinones

Popis výsledku v původním jazyce

Quinones are compounds frequently contained in medicinal plants used for the treatment of inflammatory diseases. Therefore, the impact of plant-derived quinones on the arachidonic acid metabolic pathway is worthy of investigation. In this study, twenty-three quinone compounds of plant origin were tested in vitro for their potential to inhibit leukotriene B-4 (LTB4) biosynthesis in activated human neutrophil granulocytes with 5-lipoxygenase (5-LOX) activity. The benzoquinones primin (3) and thymohydroquinone (4) (IC50 = 4.0 and 4.1 mu M, respectively) showed activity comparable with the reference inhibitor zileuton (IC50 = 4.1 mu M). Moderate activity was observed for the benzoquinone thymoquinone (2) (IC50 = 18.2 mu M) and the naphthoquinone shikonin (1) (IC50 = 24.3 mu M). The anthraquinone emodin and the naphthoquinone plumbagin (5) displayed only weak activities (IC50 > 50 mu M). The binding modes of the active compounds were further evaluated in silico by molecular docking to the h

Název v anglickém jazyce

Inhibition of In Vitro Leukotriene B-4 Biosynthesis in Human Neutrophil Granulocytes and Docking Studies of Natural Quinones

Popis výsledku anglicky

Quinones are compounds frequently contained in medicinal plants used for the treatment of inflammatory diseases. Therefore, the impact of plant-derived quinones on the arachidonic acid metabolic pathway is worthy of investigation. In this study, twenty-three quinone compounds of plant origin were tested in vitro for their potential to inhibit leukotriene B-4 (LTB4) biosynthesis in activated human neutrophil granulocytes with 5-lipoxygenase (5-LOX) activity. The benzoquinones primin (3) and thymohydroquinone (4) (IC50 = 4.0 and 4.1 mu M, respectively) showed activity comparable with the reference inhibitor zileuton (IC50 = 4.1 mu M). Moderate activity was observed for the benzoquinone thymoquinone (2) (IC50 = 18.2 mu M) and the naphthoquinone shikonin (1) (IC50 = 24.3 mu M). The anthraquinone emodin and the naphthoquinone plumbagin (5) displayed only weak activities (IC50 > 50 mu M). The binding modes of the active compounds were further evaluated in silico by molecular docking to the h

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Natural product communications

ISSN

1934-578X

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

105-108

Kód UT WoS článku

000314617200027

EID výsledku v databázi Scopus

—