Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190704" target="_blank" >RIV/00216208:11310/13:10190704 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/13:00398129

Výsledek na webu

<a href="http://dx.doi.org/10.1091/mbc.E12-06-0492" target="_blank" >http://dx.doi.org/10.1091/mbc.E12-06-0492</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1091/mbc.E12-06-0492" target="_blank" >10.1091/mbc.E12-06-0492</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana

Popis výsledku v původním jazyce

The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as an exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize to secretory-active regions of the plasma membrane, exemplified by the outer domain of Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized the dynamics of exocyst subunits at this domain. The subunits colocalized in defined foci at the plasma membrane, distinct from endocytic sites. Exocyst foci were independent of cytoskeleton, although prolonged actin disruption led to changes in exocyst localization. Exocyst foci partially overlapped with vesicles visualized by VAMP721 v-SNARE, but the majority of the foci represent sites without vesicles, as indicated by electron microscopy and drug treatments, supporting the concept of the exocyst functioning as a dynamic particle. We observe

Název v anglickém jazyce

Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana

Popis výsledku anglicky

The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as an exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize to secretory-active regions of the plasma membrane, exemplified by the outer domain of Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized the dynamics of exocyst subunits at this domain. The subunits colocalized in defined foci at the plasma membrane, distinct from endocytic sites. Exocyst foci were independent of cytoskeleton, although prolonged actin disruption led to changes in exocyst localization. Exocyst foci partially overlapped with vesicles visualized by VAMP721 v-SNARE, but the majority of the foci represent sites without vesicles, as indicated by electron microscopy and drug treatments, supporting the concept of the exocyst functioning as a dynamic particle. We observe

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP305%2F11%2F1629" target="_blank" >GAP305/11/1629: Funkce rostlinného poutacího komplexu exocyst v exocytóze, buněčném dělení a biogenezi buněčné stěny.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology of the Cell

ISSN

1059-1524

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

510-520

Kód UT WoS článku

000321117800008

EID výsledku v databázi Scopus

—