32P-Postlabeling Analysis of DNA Adducts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190859" target="_blank" >RIV/00216208:11310/13:10190859 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-62703-529-3_21" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-529-3_21</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-529-3_21" target="_blank" >10.1007/978-1-62703-529-3_21</a>

Jazyk výsledku

angličtina

Název v původním jazyce

32P-Postlabeling Analysis of DNA Adducts

Popis výsledku v původním jazyce

32P-Postlabeling analysis is an ultrasensitive method for the detection and quantitation of DNA adducts and covalent modifications of the DNA. It consists of four main steps: (1) enzymatic digestion of DNA to 3'-monophosphate nucleosides; (2) enrichmentof the adducts; (3) 5'OH-labeling of the adducts by T4 polynucleotide kinase-catalyzed transfer of 32P-orthophosphate from [?-32P]ATP; and (4) chromato-graphic or electrophoretic separation of labeled adducts and detection and quantification by means oftheir radioactive decay. The assay requires only micrograms of DNA and is capable of detecting adduct levels as low as one adduct in 109-1010 normal nucleotides. It is applicable to a wide range of investigations in human, animal, and in vitro studies including monitoring exposure to environmental or occupational carcinogens, determining whether a chemical or a complex mixture has genotoxic properties, elucidation of the toxicological pathways of carcinogens, and monitoring DNA repair.

Název v anglickém jazyce

32P-Postlabeling Analysis of DNA Adducts

Popis výsledku anglicky

32P-Postlabeling analysis is an ultrasensitive method for the detection and quantitation of DNA adducts and covalent modifications of the DNA. It consists of four main steps: (1) enzymatic digestion of DNA to 3'-monophosphate nucleosides; (2) enrichmentof the adducts; (3) 5'OH-labeling of the adducts by T4 polynucleotide kinase-catalyzed transfer of 32P-orthophosphate from [?-32P]ATP; and (4) chromato-graphic or electrophoretic separation of labeled adducts and detection and quantification by means oftheir radioactive decay. The assay requires only micrograms of DNA and is capable of detecting adduct levels as low as one adduct in 109-1010 normal nucleotides. It is applicable to a wide range of investigations in human, animal, and in vitro studies including monitoring exposure to environmental or occupational carcinogens, determining whether a chemical or a complex mixture has genotoxic properties, elucidation of the toxicological pathways of carcinogens, and monitoring DNA repair.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Genotoxicity Assessment - Methods and Protocols

ISBN

978-1-62703-528-6

Počet stran výsledku

13

Strana od-do

389-401

Počet stran knihy

463

Název nakladatele

Humana Press Inc.

Místo vydání

New York, US

Kód UT WoS kapitoly

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