In vivo exposure to 17 beta-estradiol triggers premature sperm capacitation in cauda epididymis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191736" target="_blank" >RIV/00216208:11310/13:10191736 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/13:00388348

Výsledek na webu

<a href="http://dx.doi.org/10.1530/REP-12-0472" target="_blank" >http://dx.doi.org/10.1530/REP-12-0472</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-12-0472" target="_blank" >10.1530/REP-12-0472</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo exposure to 17 beta-estradiol triggers premature sperm capacitation in cauda epididymis

Popis výsledku v původním jazyce

Estrogens play a crucial role in spermatogenesis and estrogen receptor a knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation in vitro; however this may lead to the reduction of reproductive potential due to the decreased ability of these sperm to undergo the acrosome reaction. To date the in vivo effect of estrogens on the ability of sperm to capacitate has not been investigated. Therefore, in this study, we exposed mice (n=24) to 17 beta-estradiol (E-2) at the concentration of 20 ng/ml either during puberty from the fourth to seventh week of age (n=8), or continuously from birth for a period of 12 weeks (n=8) at which age the animals from both groups were killed. The capacitationstatus of epididymal and testicular sperm was analysed by tyrosine phosphorylation (TyrP) antibody (immunofluorescence and western blot) and chlortetracycline (CTC) assay. According to our results, in vivo exposure to increased E-2 concen

Název v anglickém jazyce

In vivo exposure to 17 beta-estradiol triggers premature sperm capacitation in cauda epididymis

Popis výsledku anglicky

Estrogens play a crucial role in spermatogenesis and estrogen receptor a knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation in vitro; however this may lead to the reduction of reproductive potential due to the decreased ability of these sperm to undergo the acrosome reaction. To date the in vivo effect of estrogens on the ability of sperm to capacitate has not been investigated. Therefore, in this study, we exposed mice (n=24) to 17 beta-estradiol (E-2) at the concentration of 20 ng/ml either during puberty from the fourth to seventh week of age (n=8), or continuously from birth for a period of 12 weeks (n=8) at which age the animals from both groups were killed. The capacitationstatus of epididymal and testicular sperm was analysed by tyrosine phosphorylation (TyrP) antibody (immunofluorescence and western blot) and chlortetracycline (CTC) assay. According to our results, in vivo exposure to increased E-2 concen

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction

ISSN

1470-1626

e-ISSN

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Svazek periodika

145

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

255-263

Kód UT WoS článku

000317562900007

EID výsledku v databázi Scopus

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