Preparation of silybin and isosilybin sulfates by sulfotransferase from Desulfitobacterium hafniense

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10192041" target="_blank" >RIV/00216208:11310/13:10192041 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/13:00395307 RIV/61388963:_____/13:00395307

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.molcatb.2012.12.005" target="_blank" >http://dx.doi.org/10.1016/j.molcatb.2012.12.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molcatb.2012.12.005" target="_blank" >10.1016/j.molcatb.2012.12.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of silybin and isosilybin sulfates by sulfotransferase from Desulfitobacterium hafniense

Popis výsledku v původním jazyce

Flavonolignans silybin and isosilybin are major components of silymarin complex isolated from seeds of the milk thistle (Silybum marianum) featuring strong antioxidant and hepatoprotective effects, and also anticancer, chemoprotective, dermatoprotectiveand hypocholesterolemic activities. Natural silybin and isosilybin are mixtures of diastereoisomers: silybin/isosilybin A (1a, 1b) and silybin/isosilybin B (2a, 2b). The metabolism of these compounds is supposed to be strongly linked to Phase II of biotransformation and the respective conjugates are rapidly excreted in bile and urine. The aim of this study was to obtain optically pure sulfated metabolites of both silybins and isosilybins. Aryl-sulfate sulfotransferase (EC 2.8.2.22) from Desulfitobacterium hafniense was found to be a highly effective tool for the regiospecific enzymatic synthesis of silybin A-20-O-sulfate, silybin B-20-O-sulfate, isosilybin A-20-O-sulfate and isosilybin B-20-O-sulfate providing nearly quantitative yields

Název v anglickém jazyce

Preparation of silybin and isosilybin sulfates by sulfotransferase from Desulfitobacterium hafniense

Popis výsledku anglicky

Flavonolignans silybin and isosilybin are major components of silymarin complex isolated from seeds of the milk thistle (Silybum marianum) featuring strong antioxidant and hepatoprotective effects, and also anticancer, chemoprotective, dermatoprotectiveand hypocholesterolemic activities. Natural silybin and isosilybin are mixtures of diastereoisomers: silybin/isosilybin A (1a, 1b) and silybin/isosilybin B (2a, 2b). The metabolism of these compounds is supposed to be strongly linked to Phase II of biotransformation and the respective conjugates are rapidly excreted in bile and urine. The aim of this study was to obtain optically pure sulfated metabolites of both silybins and isosilybins. Aryl-sulfate sulfotransferase (EC 2.8.2.22) from Desulfitobacterium hafniense was found to be a highly effective tool for the regiospecific enzymatic synthesis of silybin A-20-O-sulfate, silybin B-20-O-sulfate, isosilybin A-20-O-sulfate and isosilybin B-20-O-sulfate providing nearly quantitative yields

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Catalysis - B Enzymatic

ISSN

1381-1177

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

May (2013)

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

24-27

Kód UT WoS článku

000315552300004

EID výsledku v databázi Scopus

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