DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10194130" target="_blank" >RIV/00216208:11310/13:10194130 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)

Popis výsledku v původním jazyce

Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms.

Název v anglickém jazyce

DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)

Popis výsledku anglicky

Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-07210P" target="_blank" >GP13-07210P: Diverzita v rámci druhového komplexu Frustulia rhomboides (Bacillariophyceae): multidisciplinární přístup.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nova Hedwigia

ISSN

0029-5035

e-ISSN

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Svazek periodika

Neuveden

Číslo periodika v rámci svazku

142

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

147-162

Kód UT WoS článku

000325381600013

EID výsledku v databázi Scopus

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