De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10209509" target="_blank" >RIV/00216208:11310/13:10209509 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/13:00391726

Výsledek na webu

<a href="http://dx.doi.org/10.1002/cbic.201200684" target="_blank" >http://dx.doi.org/10.1002/cbic.201200684</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cbic.201200684" target="_blank" >10.1002/cbic.201200684</a>

Jazyk výsledku

angličtina

Název v původním jazyce

De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Popis výsledku v původním jazyce

De novo biosynthesis of male sex pheromone from two bumblebee species (Bombus terrestris and Bombus lucorum) was studied by using in vitro incubations of labial glands (LGs) with radioactive [1,2-C-14] acetate and deuterated [D-3]acetate. The labeled substrate was incorporated into several types of compounds, such as terpenic alcohols, fatty acids, esters, and hydrocarbons. A similar incubation of [1,2-C-14] acetate with fat bodies (FB) led to the formation of fatty acids, triacylglycerols (TAG), and hydrocarbons. To support the results from in vitro incubations, PCR analysis of fatty acid synthase (FAS) transcripts in LG and FB was performed. Relative quantification of FAS transcription levels revealed that the abundance of mRNA from the FAS gene is afunction of the age of B. terrestris males. A comparison of the relative FAS mRNA gene transcription level in FB and LGs of B. terrestris and B. lucorum males proved that high biosynthetic activity takes place in the LGs of both species.

Název v anglickém jazyce

De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Popis výsledku anglicky

De novo biosynthesis of male sex pheromone from two bumblebee species (Bombus terrestris and Bombus lucorum) was studied by using in vitro incubations of labial glands (LGs) with radioactive [1,2-C-14] acetate and deuterated [D-3]acetate. The labeled substrate was incorporated into several types of compounds, such as terpenic alcohols, fatty acids, esters, and hydrocarbons. A similar incubation of [1,2-C-14] acetate with fat bodies (FB) led to the formation of fatty acids, triacylglycerols (TAG), and hydrocarbons. To support the results from in vitro incubations, PCR analysis of fatty acid synthase (FAS) transcripts in LG and FB was performed. Relative quantification of FAS transcription levels revealed that the abundance of mRNA from the FAS gene is afunction of the age of B. terrestris males. A comparison of the relative FAS mRNA gene transcription level in FB and LGs of B. terrestris and B. lucorum males proved that high biosynthetic activity takes place in the LGs of both species.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F09%2F1446" target="_blank" >GA203/09/1446: Studium enzymů podílejících se na biosyntéze feromonů modelových druhů čmeláků.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chembiochem : a European journal of chemical biology

ISSN

1439-4227

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

361-371

Kód UT WoS článku

000315491700012

EID výsledku v databázi Scopus

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