Morphological analysis of embryonic cerebellar grafts in SCA2 mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10210639" target="_blank" >RIV/00216208:11310/14:10210639 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/14:10210639

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0304394013010203#" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0304394013010203#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.neulet.2013.11.020" target="_blank" >10.1016/j.neulet.2013.11.020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Morphological analysis of embryonic cerebellar grafts in SCA2 mice

Popis výsledku v původním jazyce

SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The

Název v anglickém jazyce

Morphological analysis of embryonic cerebellar grafts in SCA2 mice

Popis výsledku anglicky

SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/LD12056" target="_blank" >LD12056: Neurotransplantační terapie u myšího modelu spinocerebelární ataxie typu 2</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neuroscience Letters

ISSN

0304-3940

e-ISSN

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Svazek periodika

558

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

5

Strana od-do

154-158

Kód UT WoS článku

000338481300029

EID výsledku v databázi Scopus

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