Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10227341" target="_blank" >RIV/00216208:11310/14:10227341 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pntd.0002709" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0002709</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pntd.0002709" target="_blank" >10.1371/journal.pntd.0002709</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

Popis výsledku v původním jazyce

Two cDNA libraries were constructed from female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Phylogenetic analysis were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We demonstrated that there is no significantdifference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen and Melka Werer. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland

Název v anglickém jazyce

Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

Popis výsledku anglicky

Two cDNA libraries were constructed from female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Phylogenetic analysis were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We demonstrated that there is no significantdifference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen and Melka Werer. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-05292S" target="_blank" >GA13-05292S: Proteiny slinných žlaz Phlebotomus orientalis a protilátková odpověď pobodaných hostitelů</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS Neglected Tropical Diseases [online]

ISSN

1935-2735

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

19

Strana od-do

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Kód UT WoS článku

000332017500009

EID výsledku v databázi Scopus

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