Ultrastructure and molecular phylogeny of four new species of monoxenous trypanosomatids from flies (Diptera: Brachycera) with redefinition of the genus Wallaceina

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10227350" target="_blank" >RIV/00216208:11310/14:10227350 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/14:00429469 RIV/61988987:17310/14:A1501CH5 RIV/60076658:12310/14:43887185

Výsledek na webu

<a href="http://dx.doi.org/10.14411/fp.2014.023" target="_blank" >http://dx.doi.org/10.14411/fp.2014.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/fp.2014.023" target="_blank" >10.14411/fp.2014.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastructure and molecular phylogeny of four new species of monoxenous trypanosomatids from flies (Diptera: Brachycera) with redefinition of the genus Wallaceina

Popis výsledku v původním jazyce

Four new species of monoxenous kinetoplastid parasites are described from Brachycera flies, namely Wallaceina raviniae Votypka et Lukes, 2014 and Crithidia otongatchiensis Votypka et Lukes, 2014 from Ecuador, Leptomonas moramango Votypka et Luke, 2014 from Madagascar, and Crithidia pragensis Votypka, Klepetkova et Lukes, 2014 from the Czech Republic. The new species are described here based on sequence analysis of their spliced leader (SL) RNA, glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) and small subunit (SSU) rRNA genes, as well as their morphology and ultrastructure. High-pressure freezing and Bernhard's EDTA regressive staining, used for the first time for monoxenous (one host) trypanosomatids, revealed the presence of viral particles with cytosolic localization in one and unique mitochondrial localization in another species. In accordance with previous observations, our results emphasize a discrepancy between morphology and molecular taxonomy of the family Trypano

Název v anglickém jazyce

Ultrastructure and molecular phylogeny of four new species of monoxenous trypanosomatids from flies (Diptera: Brachycera) with redefinition of the genus Wallaceina

Popis výsledku anglicky

Four new species of monoxenous kinetoplastid parasites are described from Brachycera flies, namely Wallaceina raviniae Votypka et Lukes, 2014 and Crithidia otongatchiensis Votypka et Lukes, 2014 from Ecuador, Leptomonas moramango Votypka et Luke, 2014 from Madagascar, and Crithidia pragensis Votypka, Klepetkova et Lukes, 2014 from the Czech Republic. The new species are described here based on sequence analysis of their spliced leader (SL) RNA, glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) and small subunit (SSU) rRNA genes, as well as their morphology and ultrastructure. High-pressure freezing and Bernhard's EDTA regressive staining, used for the first time for monoxenous (one host) trypanosomatids, revealed the presence of viral particles with cytosolic localization in one and unique mitochondrial localization in another species. In accordance with previous observations, our results emphasize a discrepancy between morphology and molecular taxonomy of the family Trypano

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Parasitologica

ISSN

0015-5683

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

16

Strana od-do

97-112

Kód UT WoS článku

000335539800001

EID výsledku v databázi Scopus

—