Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne as multi-redox centre DNA label at mercury meniscus modified silver solid amalgam electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10271946" target="_blank" >RIV/00216208:11310/14:10271946 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00435150 RIV/61388963:_____/14:00435150 RIV/00216224:14740/14:00073589

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2013.09.147" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2013.09.147</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2013.09.147" target="_blank" >10.1016/j.electacta.2013.09.147</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne as multi-redox centre DNA label at mercury meniscus modified silver solid amalgam electrode

Popis výsledku v původním jazyce

A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide (dC(DNPH)MP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Adsorptive and transfer stripping techniques offered sensitive detection of the studied compounds at submicromolar levels with low sample volume consumption (about 0.5 mu l), respectively. The ssDNA-DNPH gives three specific cathodic signals related to the reduction of the redox label. All these signals advantageously appear at significantly lessnegative potentials than unmodified nucleic acid and they may be optionally switched on/off by properly adjusted potential windows and scan rates. This opportunity increases signal response diversity and selectivity in DNA sensing. Mechan

Název v anglickém jazyce

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne as multi-redox centre DNA label at mercury meniscus modified silver solid amalgam electrode

Popis výsledku anglicky

A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide (dC(DNPH)MP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Adsorptive and transfer stripping techniques offered sensitive detection of the studied compounds at submicromolar levels with low sample volume consumption (about 0.5 mu l), respectively. The ssDNA-DNPH gives three specific cathodic signals related to the reduction of the redox label. All these signals advantageously appear at significantly lessnegative potentials than unmodified nucleic acid and they may be optionally switched on/off by properly adjusted potential windows and scan rates. This opportunity increases signal response diversity and selectivity in DNA sensing. Mechan

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica Acta

ISSN

0013-4686

e-ISSN

—

Svazek periodika

126

Číslo periodika v rámci svazku

20 April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

122-131

Kód UT WoS článku

000336340900016

EID výsledku v databázi Scopus

—