Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp by means of antibiotic inactivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10281654" target="_blank" >RIV/00216208:11310/14:10281654 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/1574-6968.12442" target="_blank" >http://dx.doi.org/10.1111/1574-6968.12442</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1574-6968.12442" target="_blank" >10.1111/1574-6968.12442</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp by means of antibiotic inactivation

Popis výsledku v původním jazyce

Volatiles produced by bacterial cultures are known to induce regulatory and metabolic alterations in nearby con-specific or heterospecific bacteria, resulting in phenotypic changes including acquisition of antibiotic resistance. We observed unhindered growth of ampicillin-sensitive Serratia rubidaea and S.marcescens on ampicillin-containing media, when exposed to volatiles produced by dense bacterial growth. However, this phenomenon appeared to result from pH increase in the medium caused by bacterial volatiles rather than alterations in the properties of the bacterial cultures, as alkalization of ampicillin-containing culture media to pH 8.5 by ammonia or Tris exhibited the same effects, while pretreatment of bacterial cultures under the same conditions prior to antibiotic exposure did not increase ampicillin resistance. Ampicillin was readily inactivated at pH 8.5, suggesting that observed bacterial growth results from metabolic alteration of the medium, rather than an active change

Název v anglickém jazyce

Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp by means of antibiotic inactivation

Popis výsledku anglicky

Volatiles produced by bacterial cultures are known to induce regulatory and metabolic alterations in nearby con-specific or heterospecific bacteria, resulting in phenotypic changes including acquisition of antibiotic resistance. We observed unhindered growth of ampicillin-sensitive Serratia rubidaea and S.marcescens on ampicillin-containing media, when exposed to volatiles produced by dense bacterial growth. However, this phenomenon appeared to result from pH increase in the medium caused by bacterial volatiles rather than alterations in the properties of the bacterial cultures, as alkalization of ampicillin-containing culture media to pH 8.5 by ammonia or Tris exhibited the same effects, while pretreatment of bacterial cultures under the same conditions prior to antibiotic exposure did not increase ampicillin resistance. Ampicillin was readily inactivated at pH 8.5, suggesting that observed bacterial growth results from metabolic alteration of the medium, rather than an active change

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-24275S" target="_blank" >GA13-24275S: Biosféra jako sémiosféra: život jako sebeinterpretace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Svazek periodika

354

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

126-132

Kód UT WoS článku

000335987800007

EID výsledku v databázi Scopus

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