Resistance to the crayfish plague pathogen, Aphanomyces astaci, in two freshwater shrimps

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283386" target="_blank" >RIV/00216208:11310/14:10283386 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/14:43886861

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jip.2014.07.004" target="_blank" >http://dx.doi.org/10.1016/j.jip.2014.07.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jip.2014.07.004" target="_blank" >10.1016/j.jip.2014.07.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Resistance to the crayfish plague pathogen, Aphanomyces astaci, in two freshwater shrimps

Popis výsledku v původním jazyce

Aphanomyces astaci, the causal agent of the crayfish plague, has recently been confirmed to infect also freshwater-inhabiting crabs. We experimentally tested the resistance of freshwater shrimps, another important decapod group inhabiting freshwaters, tothis pathogen. We exposed individuals of two Asian shrimp species, Macro brachium dayanum and Neocaridina davidi, to zoospores of the pathogen strain isolated from Procambarus clarkii, a known A. astaci carrier likely to get into contact with shrimps. The shrimps were kept in separate vessels up to seven weeks; exuviae and randomly chosen individuals were sampled throughout the experiment. Shrimp bodies and exuviae were tested for A. astaci presence by a species-specific quantitative PCR. The results were compared with amounts of A. astaci DNA in an inert substrate to distinguish potential pathogen growth in live specimens from persisting spores or environmental DNA attached to their surface. In contrast to susceptible crayfish Astacus

Název v anglickém jazyce

Resistance to the crayfish plague pathogen, Aphanomyces astaci, in two freshwater shrimps

Popis výsledku anglicky

Aphanomyces astaci, the causal agent of the crayfish plague, has recently been confirmed to infect also freshwater-inhabiting crabs. We experimentally tested the resistance of freshwater shrimps, another important decapod group inhabiting freshwaters, tothis pathogen. We exposed individuals of two Asian shrimp species, Macro brachium dayanum and Neocaridina davidi, to zoospores of the pathogen strain isolated from Procambarus clarkii, a known A. astaci carrier likely to get into contact with shrimps. The shrimps were kept in separate vessels up to seven weeks; exuviae and randomly chosen individuals were sampled throughout the experiment. Shrimp bodies and exuviae were tested for A. astaci presence by a species-specific quantitative PCR. The results were compared with amounts of A. astaci DNA in an inert substrate to distinguish potential pathogen growth in live specimens from persisting spores or environmental DNA attached to their surface. In contrast to susceptible crayfish Astacus

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Invertebrate Pathology

ISSN

0022-2011

e-ISSN

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Svazek periodika

121

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

97-104

Kód UT WoS článku

000341284400016

EID výsledku v databázi Scopus

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