Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10293606" target="_blank" >RIV/00216208:11310/14:10293606 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/14:00428816 RIV/67985939:_____/14:00429493

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.soilbio.2013.08.027" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2013.08.027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2013.08.027" target="_blank" >10.1016/j.soilbio.2013.08.027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?

Popis výsledku v původním jazyce

Different primer systems have been developed to characterize arbuscular mycorrhizal fungal (AMF) communities; however, a direct comparison of their specificity, potential to describe diversity and representation of different phylogenetic lineages is lacking. Using seven root samples, we compared four routinely used AMF-specific primer systems for nuclear ribosomal DNA covering i) the partial small subunit (SSU), ii) the partial large subunit (LSU), iii) the partial SSU and internal transcribed spacer (ITS; "Redecker") and iv) the partial SSU ITS partial LSU region ("Kruger"). In addition, a new primer combination v) covering the ITS2 region (ITS2) was included in the comparison. The "Kruger" primers tended to yield the highest AMF diversity and showeda significantly higher Shannon diversity index than the SSU primers. We found a strong bias towards the Glomeraceae in the LSU and SSU primer systems and differences in the composition of AMF communities based on the "Redecker" primer sys

Název v anglickém jazyce

Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?

Popis výsledku anglicky

Different primer systems have been developed to characterize arbuscular mycorrhizal fungal (AMF) communities; however, a direct comparison of their specificity, potential to describe diversity and representation of different phylogenetic lineages is lacking. Using seven root samples, we compared four routinely used AMF-specific primer systems for nuclear ribosomal DNA covering i) the partial small subunit (SSU), ii) the partial large subunit (LSU), iii) the partial SSU and internal transcribed spacer (ITS; "Redecker") and iv) the partial SSU ITS partial LSU region ("Kruger"). In addition, a new primer combination v) covering the ITS2 region (ITS2) was included in the comparison. The "Kruger" primers tended to yield the highest AMF diversity and showeda significantly higher Shannon diversity index than the SSU primers. We found a strong bias towards the Glomeraceae in the LSU and SSU primer systems and differences in the composition of AMF communities based on the "Redecker" primer sys

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

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Svazek periodika

68

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

482-493

Kód UT WoS článku

000329536200053

EID výsledku v databázi Scopus

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