Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10293513" target="_blank" >RIV/00216208:11310/15:10293513 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/15:10293513 RIV/00064165:_____/15:10293513

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002" target="_blank" >http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002" target="_blank" >10.1016/j.clinbiochem.2014.12.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS

Popis výsledku v původním jazyce

Objectives: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicologypurposes. Design and methods: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. Results: The assay was proved to be quadratic in the range of 50 to 5000 mg L-1 with adequate accuracy (96.3-105.8%) and precision (CV {= 8.9%). Conclusion: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R-2 0.9933 for ethylene glycol and 0.9943 for glycolic acid). (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier

Název v anglickém jazyce

Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS

Popis výsledku anglicky

Objectives: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicologypurposes. Design and methods: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. Results: The assay was proved to be quadratic in the range of 50 to 5000 mg L-1 with adequate accuracy (96.3-105.8%) and precision (CV {= 8.9%). Conclusion: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R-2 0.9933 for ethylene glycol and 0.9943 for glycolic acid). (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Biochemistry

ISSN

0009-9120

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

3

Strana od-do

189-191

Kód UT WoS článku

000348703300019

EID výsledku v databázi Scopus

2-s2.0-84919499914