Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/?PIX Proteins and Calcium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10293952" target="_blank" >RIV/00216208:11310/15:10293952 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/15:00455873

Výsledek na webu

<a href="http://dx.doi.org/10.4049/jimmunol.1402459" target="_blank" >http://dx.doi.org/10.4049/jimmunol.1402459</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4049/jimmunol.1402459" target="_blank" >10.4049/jimmunol.1402459</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/?PIX Proteins and Calcium

Popis výsledku v původním jazyce

Ag-mediated activation of mast cells initiates signaling events leading to Ca(2+) response, release of allergic mediators from cytoplasmic granules, and synthesis of cytokines and chemokines. Although microtubule rearrangement during activation has beendescribed, the molecular mechanisms that control their remodeling are largely unknown. Microtubule nucleation is mediated by complexes that are formed by ?-tubulin and ?-tubulin complex proteins. In this study, we report that, in bone marrow-derived mastcells (BMMCs), ?-tubulin interacts with p21-activated kinase interacting exchange factor ? (?PIX) and G protein-coupled receptor kinase-interacting protein (GIT)1. Microtubule regrowth experiments showed that the depletion of ?PIX in BMMCs stimulated microtubule nucleation, whereas depletion of GIT1 led to the inhibition of nucleation compared with control cells. Phenotypic rescue experiments confirmed that ?PIX and GIT1 represent negative and positive regulators of microtubule nucleati

Název v anglickém jazyce

Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/?PIX Proteins and Calcium

Popis výsledku anglicky

Ag-mediated activation of mast cells initiates signaling events leading to Ca(2+) response, release of allergic mediators from cytoplasmic granules, and synthesis of cytokines and chemokines. Although microtubule rearrangement during activation has beendescribed, the molecular mechanisms that control their remodeling are largely unknown. Microtubule nucleation is mediated by complexes that are formed by ?-tubulin and ?-tubulin complex proteins. In this study, we report that, in bone marrow-derived mastcells (BMMCs), ?-tubulin interacts with p21-activated kinase interacting exchange factor ? (?PIX) and G protein-coupled receptor kinase-interacting protein (GIT)1. Microtubule regrowth experiments showed that the depletion of ?PIX in BMMCs stimulated microtubule nucleation, whereas depletion of GIT1 led to the inhibition of nucleation compared with control cells. Phenotypic rescue experiments confirmed that ?PIX and GIT1 represent negative and positive regulators of microtubule nucleati

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Immunology

ISSN

0022-1767

e-ISSN

—

Svazek periodika

194

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

4099-4111

Kód UT WoS článku

000353727400009

EID výsledku v databázi Scopus

2-s2.0-84928484964