Acute morphine affects the rat circadian clock via rhythms of phosphorylated ERK1/2 and GSK3 kinases and Per1 expression in the rat suprachiasmatic nucleus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297866" target="_blank" >RIV/00216208:11310/15:10297866 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/bph.13152" target="_blank" >http://dx.doi.org/10.1111/bph.13152</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/bph.13152" target="_blank" >10.1111/bph.13152</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acute morphine affects the rat circadian clock via rhythms of phosphorylated ERK1/2 and GSK3 kinases and Per1 expression in the rat suprachiasmatic nucleus

Popis výsledku v původním jazyce

Background and PurposeOpioids affect the circadian clock and may change the timing of many physiological processes. This study was undertaken to investigate the daily changes in sensitivity of the circadian pacemaker to an analgesic dose of morphine, andto uncover a possible interplay between circadian and opioid signalling. Experimental ApproachA time-dependent effect of morphine (1mgkg(-1), i.p.) applied either during the day or during the early night was followed, and the levels of phosphorylated ERK1/2, GSK3, c-Fos and Per genes were assessed by immunohistochemistry and in situ hybridization. The effect of morphine pretreatment on light-induced pERK and c-Fos was examined, and day/night difference in activity of opioid receptors was evaluated by [S-35]-GTPS binding assay. Key ResultsMorphine stimulated a rise in pERK1/2 and pGSK3 levels in the suprachiasmatic nucleus (SCN) when applied during the day but significantly reduced both kinases when applied during the night. Morphine at

Název v anglickém jazyce

Acute morphine affects the rat circadian clock via rhythms of phosphorylated ERK1/2 and GSK3 kinases and Per1 expression in the rat suprachiasmatic nucleus

Popis výsledku anglicky

Background and PurposeOpioids affect the circadian clock and may change the timing of many physiological processes. This study was undertaken to investigate the daily changes in sensitivity of the circadian pacemaker to an analgesic dose of morphine, andto uncover a possible interplay between circadian and opioid signalling. Experimental ApproachA time-dependent effect of morphine (1mgkg(-1), i.p.) applied either during the day or during the early night was followed, and the levels of phosphorylated ERK1/2, GSK3, c-Fos and Per genes were assessed by immunohistochemistry and in situ hybridization. The effect of morphine pretreatment on light-induced pERK and c-Fos was examined, and day/night difference in activity of opioid receptors was evaluated by [S-35]-GTPS binding assay. Key ResultsMorphine stimulated a rise in pERK1/2 and pGSK3 levels in the suprachiasmatic nucleus (SCN) when applied during the day but significantly reduced both kinases when applied during the night. Morphine at

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP303%2F12%2F1137" target="_blank" >GAP303/12/1137: Úloha proteinů STAT v cirkadiánním systému potkana</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

British Journal of Pharmacology

ISSN

0007-1188

e-ISSN

—

Svazek periodika

172

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

3638-3649

Kód UT WoS článku

000356976300014

EID výsledku v databázi Scopus

2-s2.0-84929259889