Challenges of Flow-Cytometric Estimation of Nuclear Genome Size in Orchids, a Plant Group with Both Whole-Genome and Progressively Partial Endoreplication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10311922" target="_blank" >RIV/00216208:11310/15:10311922 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/15:00449202 RIV/67179843:_____/15:00449202 RIV/61389030:_____/15:00449202 RIV/60076658:12310/15:43889039 RIV/60076658:12220/15:43889039

Výsledek na webu

<a href="http://dx.doi.org/10.1002/cyto.a.22681" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22681</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.a.22681" target="_blank" >10.1002/cyto.a.22681</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Challenges of Flow-Cytometric Estimation of Nuclear Genome Size in Orchids, a Plant Group with Both Whole-Genome and Progressively Partial Endoreplication

Popis výsledku v původním jazyce

Nuclear genome size is an inherited quantitative trait of eukaryotic organisms with both practical and biological consequences. A detailed analysis of major families is a promising approach to fully understand the biological meaning of the extensive variation in genome size in plants. Although Orchidaceae accounts for similar to 10% of the angiosperm diversity, the knowledge of patterns and dynamics of their genome size is limited, in part due to difficulties in flow cytometric analyses. Cells in various somatic tissues of orchids undergo extensive endoreplication, either whole-genome or partial, and the G1-phase nuclei with 2C DNA amounts may be lacking, resulting in overestimated genome size values. Interpretation of DNA content histograms is particularly challenging in species with progressively partial endoreplication, in which the ratios between the positions of two neighboring DNA peaks are lower than two. In order to assess distributions of nuclear DNA amounts and identify tissu

Název v anglickém jazyce

Challenges of Flow-Cytometric Estimation of Nuclear Genome Size in Orchids, a Plant Group with Both Whole-Genome and Progressively Partial Endoreplication

Popis výsledku anglicky

Nuclear genome size is an inherited quantitative trait of eukaryotic organisms with both practical and biological consequences. A detailed analysis of major families is a promising approach to fully understand the biological meaning of the extensive variation in genome size in plants. Although Orchidaceae accounts for similar to 10% of the angiosperm diversity, the knowledge of patterns and dynamics of their genome size is limited, in part due to difficulties in flow cytometric analyses. Cells in various somatic tissues of orchids undergo extensive endoreplication, either whole-genome or partial, and the G1-phase nuclei with 2C DNA amounts may be lacking, resulting in overestimated genome size values. Interpretation of DNA content histograms is particularly challenging in species with progressively partial endoreplication, in which the ratios between the positions of two neighboring DNA peaks are lower than two. In order to assess distributions of nuclear DNA amounts and identify tissu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry Part A

ISSN

1552-4922

e-ISSN

—

Svazek periodika

87A

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

958-966

Kód UT WoS článku

000363042900011

EID výsledku v databázi Scopus

2-s2.0-84942369482