Bacteria detected in the honeybee parasitic mite Varroa destructor collected from beehive winter debris
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10312581" target="_blank" >RIV/00216208:11310/15:10312581 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00027006:_____/15:00003288
Výsledek na webu
<a href="http://dx.doi.org/10.1111/jam.12899" target="_blank" >http://dx.doi.org/10.1111/jam.12899</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jam.12899" target="_blank" >10.1111/jam.12899</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Bacteria detected in the honeybee parasitic mite Varroa destructor collected from beehive winter debris
Popis výsledku v původním jazyce
The winter beehive debris containing bodies of honeybee parasitic mite Varroa destructor is used for veterinary diagnostics. The Varroa sucking honeybee haemolymph serves as a reservoir of pathogens including bacteria. Worker bees can pick up pathogens from the debris during cleaning activities and spread the infection to healthy bees within the colony. The aim of this study was to detect entomopathogenic bacteria in the Varroa collected from the winter beehive debris. Culture-independent approach was used to analyse the mite-associated bacterial community. Total DNA was extracted from the samples of 10 Varroa female individuals sampled from 27 different sites in Czechia. The 16S rRNA gene was amplified using universal bacterial primers, cloned and sequenced, resulting in a set of 596 sequences representing 29 operational taxonomic units (OTU97). To confirm the presence of bacteria in Varroa, histological sections of the mites were observed. Undetermined bacteria were observed in the m
Název v anglickém jazyce
Bacteria detected in the honeybee parasitic mite Varroa destructor collected from beehive winter debris
Popis výsledku anglicky
The winter beehive debris containing bodies of honeybee parasitic mite Varroa destructor is used for veterinary diagnostics. The Varroa sucking honeybee haemolymph serves as a reservoir of pathogens including bacteria. Worker bees can pick up pathogens from the debris during cleaning activities and spread the infection to healthy bees within the colony. The aim of this study was to detect entomopathogenic bacteria in the Varroa collected from the winter beehive debris. Culture-independent approach was used to analyse the mite-associated bacterial community. Total DNA was extracted from the samples of 10 Varroa female individuals sampled from 27 different sites in Czechia. The 16S rRNA gene was amplified using universal bacterial primers, cloned and sequenced, resulting in a set of 596 sequences representing 29 operational taxonomic units (OTU97). To confirm the presence of bacteria in Varroa, histological sections of the mites were observed. Undetermined bacteria were observed in the m
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GJ - Choroby a škůdci zvířat, veterinární medicina
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Applied Microbiology
ISSN
1364-5072
e-ISSN
—
Svazek periodika
119
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
640-654
Kód UT WoS článku
000360231000004
EID výsledku v databázi Scopus
2-s2.0-84939565488