Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10312786" target="_blank" >RIV/00216208:11310/15:10312786 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/15:00455588

Výsledek na webu

<a href="http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1093706" target="_blank" >http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1093706</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15384101.2015.1093706" target="_blank" >10.1080/15384101.2015.1093706</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis

Popis výsledku v původním jazyce

Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis. Using a spectrum of different inhibitors, we showed that glycolysis is essential for the formation, and particularly, the survival of U cells. We also showed that beta-1,3-glucans that are released from the cell walls of L cells are the most likely source of carbohydrates for U cells.

Název v anglickém jazyce

Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis

Popis výsledku anglicky

Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis. Using a spectrum of different inhibitors, we showed that glycolysis is essential for the formation, and particularly, the survival of U cells. We also showed that beta-1,3-glucans that are released from the cell walls of L cells are the most likely source of carbohydrates for U cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Cycle

ISSN

1538-4101

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

3488-3497

Kód UT WoS článku

000364559400023

EID výsledku v databázi Scopus

2-s2.0-84959525672