Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314160" target="_blank" >RIV/00216208:11310/15:10314160 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0137820" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0137820</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0137820" target="_blank" >10.1371/journal.pone.0137820</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles

Popis výsledku v původním jazyce

Background Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped. Methodology/Principal Findings Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels, together with other specific defects in cell-cycle progression. Cbf11 activity is nutrient-dependent and Delta cbf11-associated defects are mitigated by inactivation of the protein kinase A (Pka1) and stress-activat

Název v anglickém jazyce

Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles

Popis výsledku anglicky

Background Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped. Methodology/Principal Findings Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels, together with other specific defects in cell-cycle progression. Cbf11 activity is nutrient-dependent and Delta cbf11-associated defects are mitigated by inactivation of the protein kinase A (Pka1) and stress-activat

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GPP305%2F12%2FP040" target="_blank" >GPP305/12/P040: Úloha proteinů CSL v udržování integrity genomu kvasinky Schizosaccharomyces pombe</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

23

Strana od-do

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Kód UT WoS článku

000361601100146

EID výsledku v databázi Scopus

2-s2.0-84947465861