Membrane activity of the pentaene macrolide didehydroroflamycoin in model lipid bilayers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314207" target="_blank" >RIV/00216208:11310/15:10314207 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/15:10314207

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbamem.2014.10.038" target="_blank" >http://dx.doi.org/10.1016/j.bbamem.2014.10.038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2014.10.038" target="_blank" >10.1016/j.bbamem.2014.10.038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Membrane activity of the pentaene macrolide didehydroroflamycoin in model lipid bilayers

Popis výsledku v původním jazyce

Didehydroroflamycoin (DDHR), a recently isolated member of the polyene macrolide family, was shown to have antibacterial and antifungal activity. However, its mechanism of action has not been investigated. Antibiotics from this family are amphiphilic; thus, they have membrane activity, their biological action is localized in the membrane, and the membrane composition and physical properties facilitate the recognition of a particular compound by the target organism. In this work, we use model lipid membranes comprised of giant unilamellar vesicles (GUVs) for a systematic study of the action of DDHR. In parallel, experiments are conducted using filipin III and amphotericin B, other members of the family, and the behavior observed for DDHR is described inthe context of that of these two heavily studied compounds. The study shows that DDHR disrupts membranes via two different mechanisms and that the involvement of these mechanisms depends on the presence of cholesterol. The leakage assays

Název v anglickém jazyce

Membrane activity of the pentaene macrolide didehydroroflamycoin in model lipid bilayers

Popis výsledku anglicky

Didehydroroflamycoin (DDHR), a recently isolated member of the polyene macrolide family, was shown to have antibacterial and antifungal activity. However, its mechanism of action has not been investigated. Antibiotics from this family are amphiphilic; thus, they have membrane activity, their biological action is localized in the membrane, and the membrane composition and physical properties facilitate the recognition of a particular compound by the target organism. In this work, we use model lipid membranes comprised of giant unilamellar vesicles (GUVs) for a systematic study of the action of DDHR. In parallel, experiments are conducted using filipin III and amphotericin B, other members of the family, and the behavior observed for DDHR is described inthe context of that of these two heavily studied compounds. The study shows that DDHR disrupts membranes via two different mechanisms and that the involvement of these mechanisms depends on the presence of cholesterol. The leakage assays

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GPP207%2F12%2FP890" target="_blank" >GPP207/12/P890: In vivo inkorporace fluorescenčních aminokyselin a ab initio modelování proteinů; univerzální nástroje pro zjištění topologie membránových proteinů.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Biomembranes

ISSN

0005-2736

e-ISSN

—

Svazek periodika

1848

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

444-452

Kód UT WoS článku

000348687500008

EID výsledku v databázi Scopus

2-s2.0-84912061294