Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314751" target="_blank" >RIV/00216208:11310/15:10314751 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/15:00449810 RIV/70883521:28110/15:43873020

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jtbi.2015.06.048" target="_blank" >http://dx.doi.org/10.1016/j.jtbi.2015.06.048</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtbi.2015.06.048" target="_blank" >10.1016/j.jtbi.2015.06.048</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio

Popis výsledku v původním jazyce

Multitemplate polymerase chain reaction (PCR) is used for preparative and analytical applications in diagnostics and research. Classical PCR and qPCR are two basic setups with many possible experimental modifications. Classical PCR is a method of choiceto obtain enough material for subsequent sophisticated applications such as construction of libraries for next-generation sequencing or high-throughput screening. Sequencing and Single Nucleotide Primer Extension (SNuPE) employ one-strand synthesis and represent a distinct variant of analytical DNA synthesis. In all these applications, maintaining the initial ratio of templates and avoiding underestimation of minority templates is desired. Here, we demonstrate that different templates can amplify independently at low template concentrations (typical in qPCR setups, in which the polymerase concentration is usually several orders of magnitude higher than the template concentration). However, rare templates can be diluted in an effort to k

Název v anglickém jazyce

Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio

Popis výsledku anglicky

Multitemplate polymerase chain reaction (PCR) is used for preparative and analytical applications in diagnostics and research. Classical PCR and qPCR are two basic setups with many possible experimental modifications. Classical PCR is a method of choiceto obtain enough material for subsequent sophisticated applications such as construction of libraries for next-generation sequencing or high-throughput screening. Sequencing and Single Nucleotide Primer Extension (SNuPE) employ one-strand synthesis and represent a distinct variant of analytical DNA synthesis. In all these applications, maintaining the initial ratio of templates and avoiding underestimation of minority templates is desired. Here, we demonstrate that different templates can amplify independently at low template concentrations (typical in qPCR setups, in which the polymerase concentration is usually several orders of magnitude higher than the template concentration). However, rare templates can be diluted in an effort to k

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Theoretical Biology

ISSN

0022-5193

e-ISSN

—

Svazek periodika

382

Číslo periodika v rámci svazku

7 October 2015

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

178-186

Kód UT WoS článku

000361085200017

EID výsledku v databázi Scopus

2-s2.0-84937848180