Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10316484" target="_blank" >RIV/00216208:11310/15:10316484 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/15:00444733

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0144861715003598" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0144861715003598</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbpol.2015.04.043" target="_blank" >10.1016/j.carbpol.2015.04.043</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization

Popis výsledku v původním jazyce

Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na beta-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth. (C) 2015 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization

Popis výsledku anglicky

Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na beta-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth. (C) 2015 Elsevier Ltd. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

—

Svazek periodika

129

Číslo periodika v rámci svazku

SEPTEMBER 2015

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

135-142

Kód UT WoS článku

000356746000018

EID výsledku v databázi Scopus

2-s2.0-84929335296