Mouse polyomavirus: Propagation, purification, quantification, and storage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10320535" target="_blank" >RIV/00216208:11310/15:10320535 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/9780471729259.mc14f01s38" target="_blank" >http://dx.doi.org/10.1002/9780471729259.mc14f01s38</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/9780471729259.mc14f01s38" target="_blank" >10.1002/9780471729259.mc14f01s38</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mouse polyomavirus: Propagation, purification, quantification, and storage

Popis výsledku v původním jazyce

Mouse polyomavirus (MPyV) is a member of the Polyomaviridae family, which comprises non-enveloped tumorigenic viruses infecting various vertebrates including humans and causing different pathogenic responses in the infected organisms. Despite the variations in host tropism and pathogenicity, the structure of the virions of these viruses is similar. The capsid, with icosahedral symmetry (o, 45 nm, T= 7d), is composed of a shell of 72 capsomeres of structural proteins, arranged around the nucleocore containing approximately 5-kbp-long circular dsDNA in complex with cellular histones. MPyV has been one of the most studied polyomaviruses and serves as a model virus for studies of the mechanisms of cell transformation and virus trafficking, and for use in nanotechnology. It can be propagated in primary mouse cells (e.g., in whole mouse embryo cells) or in mouse epithelial or fibroblast cell lines. In this unit, propagation, purification, quantification, and storage of MPyV virions are prese

Název v anglickém jazyce

Mouse polyomavirus: Propagation, purification, quantification, and storage

Popis výsledku anglicky

Mouse polyomavirus (MPyV) is a member of the Polyomaviridae family, which comprises non-enveloped tumorigenic viruses infecting various vertebrates including humans and causing different pathogenic responses in the infected organisms. Despite the variations in host tropism and pathogenicity, the structure of the virions of these viruses is similar. The capsid, with icosahedral symmetry (o, 45 nm, T= 7d), is composed of a shell of 72 capsomeres of structural proteins, arranged around the nucleocore containing approximately 5-kbp-long circular dsDNA in complex with cellular histones. MPyV has been one of the most studied polyomaviruses and serves as a model virus for studies of the mechanisms of cell transformation and virus trafficking, and for use in nanotechnology. It can be propagated in primary mouse cells (e.g., in whole mouse embryo cells) or in mouse epithelial or fibroblast cell lines. In this unit, propagation, purification, quantification, and storage of MPyV virions are prese

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-26115S" target="_blank" >GA13-26115S: Doprava genomů polyomavirů do buněčného jádra a interakce strukturních proteinů v hostitelských buňkách</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Protocols in Microbiology

ISSN

1934-8525

e-ISSN

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Svazek periodika

2015

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

26

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84949234034